What are the benefits of using a sucrose gradient in fractionation?

Hi I carried out a quick experiment in which we did use a sucrose gradient when fractioning a homogenate. Why should I have used one? I can't find a paper that tell me why only how I set one up. ThanxWhat are the benefits of using a sucrose gradient in fractionation?
A sucrose gradient allows you to separate cellular fractions that have subtle differences in their density. With a broad difference in concentration, you can separate different kinds of membrane bound organelles in a post-nuclear and/or post-mitochondrial supernatant (e.g. ER, Golgi, plasma membrane, lysosomes, vesicles).


A sucrose gradient with a sharper difference in concentration can separate these crude fractions into sub-fractions (e.g. different parts of the Golgi).





See http://www.accessexcellence.org/RC/VL/GG鈥?/a>


for a cartoon.What are the benefits of using a sucrose gradient in fractionation?
It causes less gas and bloating.

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